"Efficient selection for high-expression transfectants with a novel eukaryotic vector". ^ Niwa H, Yamamura K, Miyazaki J (December 1991)."Expression vector system based on the chicken beta-actin promoter directs efficient production of interleukin-5". ^ a b Miyazaki J, Takaki S, Araki K, Tashiro F, Tominaga A, Takatsu K, Yamamura K (July 1989)."The CMV early enhancer/chicken beta actin (CAG) promoter can be used to drive transgene expression during the differentiation of murine embryonic stem cells into vascular progenitors". ^ Alexopoulou AN, Couchman JR, Whiteford JR (January 2008)." 'Green mice' as a source of ubiquitous green cells". ^ Okabe M, Ikawa M, Kominami K, Nakanishi T, Nishimune Y (May 1997).As well as increases the expression of transgenes under low temperature conditions. Some of the CAG promoter's functions as an effective tool that increases and maintains the expression level of recombinant proteins. The 3' part of the promoter has high GC content and is thus refractory to PCR amplification. In addition to the CMV immediate early enhancer, the intron of the chicken beta actin gene contains an enhancer element, which is highly conserved among vertebrates. Īlthough the whole construct is commonly referred to as the "CAG promoter", it is not a promoter in a strict sense, as it includes a part of the transcribed sequence (the first exon and the first intron of chicken beta-actin gene) and enhancer elements. The initiation codon located at the proximal region of the second exon was disrupted by digesting with NcoI restriction enzyme and replacing the site with a HindIII linker. The resulting synthetic element was used in the pCAGGS expression vector. ( C) the cytomegalovirus (CMV) early enhancer element, ( A) the promoter, the first exon and the first intron of chicken beta-actin gene, ( G) the splice acceptor of the rabbit beta-globin gene ĬAG promoter was constructed in the lab of Dr Jun-ichi Miyazaki from the following sequences: The CAG promoter is a strong synthetic promoter frequently used to drive high levels of gene expression in mammalian expression vectors.
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